2016 Winston-Salem Open – Singles

Kevin Anderson was the defending champion, but lost in the second round to Jiří Veselý.

Pablo Carreño Busta won his maiden ATP title, defeating Roberto Bautista Agut in the final, 6–7(6–8), 7–6(7–1), 6–4.

Seeds
All seeds receive a bye into the second round. • 🇫🇷 Richard Gasquet (quarterfinals)

• 🇪🇸 Roberto Bautista Agut (final)

• Pablo Cuevas (third round)

• 🇺🇸 Steve Johnson (third round)

• Kevin Anderson (second round)

• 🇺🇸 Sam Querrey (third round)

• 🇫🇷 Gilles Simon (second round)

• 🇪🇸 Albert Ramos Viñolas (second round)

• 🇷🇸 Viktor Troicki (semifinals)

• João Sousa (second round)

• 🇮🇹 Paolo Lorenzi (second round)

• 🇷🇺 Andrey Kuznetsov (quarterfinals)

• 🇦🇷 Federico Delbonis (second round)

• 🇨🇾 Marcos Baghdatis (third round)

• 🇪🇸 Fernando Verdasco (quarterfinals)

• 🇪🇸 Pablo Carreño Busta (champion)

Seeds
• # 🇯🇵 Yoshihito Nishioka (qualified)

• # 🇲🇩 Radu Albot (qualified)

• # 🇩🇪 Michael Berrer (qualifying competition)

• # 🇦🇺 James Duckworth (qualified)

• # 🇨🇴 Alejandro Falla (first round, retired)

• # 🇮🇪 James McGee (qualified)

• # 🇬🇧 Liam Broady (qualifying competition)

• # Tim van Rijthoven (qualifying competition, lucky loser)

Qualifiers
• # 🇯🇵 Yoshihito Nishioka

• # 🇲🇩 Radu Albot

• # 🇮🇪 James McGee

• # 🇦🇺 James Duckworth

Lucky losers
• # Tim van Rijthoven