2020 Challenger Ciudad de Guayaquil – Singles

Thiago Seyboth Wild was the defending champion but chose not to defend his title.

Francisco Cerúndolo won the title after defeating Andrej Martin 6–4, 3–6, 6–2 in the final.

Seeds
• # 🇦🇷 Federico Coria (first round)

• # 🇪🇸 Roberto Carballés Baena (semifinals)

• # 🇸🇰 Andrej Martin (final)

• # 🇪🇸 Jaume Munar (quarterfinals)

• # Pedro Sousa (quarterfinals)

• # 🇵🇪 Juan Pablo Varillas (second round)

• # 🇪🇨 Emilio Gómez (first round)

• # Alejandro Tabilo (second round)