2020 I.ČLTK Prague Open – Singles

Mario Vilella Martínez was the defending champion but chose not to compete this year.

Stan Wawrinka won the title after defeating Aslan Karatsev 7–6(7–2), 6–4 in the final.

Seeds
All seeds receive a bye into the second round. • # Stan Wawrinka (champion)

• # 🇨🇿 Jiří Veselý (second round)

• # 🇫🇷 Pierre-Hugues Herbert (semifinals)

• # 🇩🇪 Philipp Kohlschreiber (third round, withdrew)

• # 🇸🇰 Jozef Kovalík (third round)

• # 🇮🇳 Sumit Nagal (quarterfinals)

• # Henri Laaksonen (quarterfinals)

• # 🇧🇾 Ilya Ivashka (second round)

• # Blaž Rola (second round)

• # 🇩🇪 Yannick Maden (third round)

• # 🇧🇪 Kimmer Coppejans (second round)

• # 🇸🇰 Martin Kližan (second round)

• # 🇫🇷 Arthur Rinderknech (second round)

• # Ernests Gulbis (third round)

• # 🇦🇹 Sebastian Ofner (second round)

• # 🇨🇦 Steven Diez (second round)