2020 Italian Open – Women's singles

Simona Halep won the women's singles tennis title at the 2020 Italian Open after the defending champion Karolína Plíšková retired from the final, with the scoreline at 6–0, 2–1. It was her first Italian Open title.

Sofia Kenin became the first top five player and reigning major champion to be defeated by a 'double bagel' (6–0, 6–0) scoreline since Lindsay Davenport defeated Maria Sharapova at the 2005 Indian Wells tournament. Kenin was defeated by Victoria Azarenka in the second round.

Seeds
The top eight seeds received a bye into the second round. • 🇷🇴 Simona Halep (champion)

• 🇨🇿 Karolína Plíšková (final, retired)

• 🇺🇸 Sofia Kenin (second round)

• 🇺🇦 Elina Svitolina (quarterfinals)

• Kiki Bertens (second round)

• Belinda Bencic (second round)

• 🇬🇧 Johanna Konta (third round)

• Petra Martić (second round)

• 🇪🇸 Garbiñe Muguruza (semifinals)

• 🇰🇿 Elena Rybakina (third round)

• 🇧🇪 Elise Mertens (quarterfinals)

• 🇨🇿 Markéta Vondroušová (semifinals)

• 🇺🇸 Alison Riske (first round)

• 🇪🇪 Anett Kontaveit (second round)

• 🇩🇪 Angelique Kerber (first round)

• Donna Vekić (first round)

Seeds
• # 🇺🇸 Bernarda Pera (moved to main draw)

• # 🇷🇺 Anna Blinkova (qualified)

• # 🇺🇸 Lauren Davis (first round)

• # Arantxa Rus (qualified)

• # 🇰🇿 Zarina Diyas (first round)

• # 🇷🇺 Daria Kasatkina (qualified)

• # 🇷🇴 Irina-Camelia Begu (qualified)

• # 🇺🇸 Christina McHale (first round)

• # 🇯🇵 Nao Hibino (first round)

• # 🇯🇵 Misaki Doi (qualified)

• # 🇷🇴 Patricia Maria Țig (withdrew, still competing in İstanbul)

• # 🇲🇪 Danka Kovinić (qualified)

• # 🇭🇺 Tímea Babos (qualifying competition)

• # 🇪🇸 Aliona Bolsova (qualified)

• # 🇨🇦 Leylah Annie Fernandez (first round)

• # 🇩🇪 Anna-Lena Friedsam (qualifying competition)

Qualifiers
• # 🇯🇵 Misaki Doi

• # 🇷🇺 Anna Blinkova

• # Kaja Juvan

• # Arantxa Rus

• # 🇪🇸 Aliona Bolsova

• # 🇷🇺 Daria Kasatkina

• # 🇷🇴 Irina-Camelia Begu

• # 🇲🇪 Danka Kovinić