2020 Prague Open – Singles

Jil Teichmann was the defending champion, but chose to participate in Lexington instead.

Simona Halep won the title, defeating Elise Mertens in the final, 6–2, 7–5.

Seeds
• #🇷🇴 Simona Halep (champion)

• # Petra Martić (second round)

• #🇧🇪 Elise Mertens (final)

• #🇺🇦 Dayana Yastremska (withdrew)

• #🇷🇺 Ekaterina Alexandrova (first round)

• #🇷🇺 Anastasia Pavlyuchenkova (first round)

• #🇨🇿 Barbora Strýcová (first round)

• #🇷🇺 Veronika Kudermetova (first round)

• # Anastasija Sevastova (first round)

Seeds
• # 🇪🇸 Aliona Bolsova (moved to main draw)

• # 🇩🇪 Anna-Lena Friedsam (first round)

• # 🇩🇪 Tamara Korpatsch (qualifying competition)

• # 🇫🇷 Océane Dodin (first round)

• # 🇷🇺 Liudmila Samsonova (first round)

• # 🇧🇾 Aliaksandra Sasnovich (second round)

• # Kaja Juvan (second round)

• # 🇧🇪 Ysaline Bonaventure (first round)

Qualifiers
• # 🇪🇬 Mayar Sherif

• # 🇺🇦 Marta Kostyuk

• # 🇷🇴 Elena-Gabriela Ruse

• # 🇺🇦 Lesia Tsurenko

Lucky losers
• # 🇦🇺 Storm Sanders

• # 🇵🇱 Magdalena Fręch

• # Leonie Küng