2020 TK Sparta Prague Open – Singles

The 2020 TK Sparta Prague Open was a women's tennis tournament played on Clay in Prague, Czech Republic, and was part of the 2020 WTA 125K series.

This was the first edition and was held from 29 August to 6 September 2020.

Kristína Kučová won the title, defeating Elisabetta Cocciaretto in the final, 6–4, 6–3.

Seeds
• 🇷🇴 Monica Niculescu (second round)

• 🇮🇹 Elisabetta Cocciaretto (final)

• 🇬🇧 Harriet Dart (first round)

• Leonie Küng (third round)

• 🇪🇸 Lara Arruabarrena (third round)

• 🇮🇹 Sara Errani (second round)

• 🇮🇹 Martina Trevisan (second round)

• 🇮🇹 Giulia Gatto-Monticone (third round)

• 🇵🇱 Magdalena Fręch (first round)

• 🇷🇴 Irina Bara (third round)

• 🇷🇴 Jaqueline Cristian (third round)

• 🇦🇷 Nadia Podoroska (semifinals)

• 🇮🇳 Ankita Raina (first round)

• 🇸🇰 Jana Čepelová (first round)

•  Lesley Pattinama Kerkhove (first round)

• 🇷🇴 Elena-Gabriela Ruse (second round)

• 🇪🇬 Mayar Sherif (third round)

• 🇸🇰 Kristína Kučová (champion)

• 🇷🇸 Olga Danilović (second round)

• 🇫🇷 Chloé Paquet (first round)

• 🇪🇸 Cristina Bucșa (third round)

• 🇺🇸 Varvara Lepchenko (first round)

• 🇬🇧 Samantha Murray Sharan (first round)

• 🇸🇰 Anna Karolína Schmiedlová (quarterfinals)

•  Indy de Vroome (first round)

•  Mariam Bolkvadze (second round)

• 🇹🇷 Çağla Büyükakçay (quarterfinals)

• 🇧🇬 Isabella Shinikova (second round)

•  Bibiane Schoofs (third round)

• 🇲🇽 Renata Zarazúa (fourth round)

• 🇮🇹 Martina Di Giuseppe (second round)

• 🇸🇰 Rebecca Šramková (second round)