2020 Western & Southern Open – Women's singles

Victoria Azarenka won the women's singles tennis title at the 2020 Cincinnati Masters via walkover, after Naomi Osaka withdrew from the final with a hamstring injury. It was Azarenka's first singles title since the 2016 Miami Open, and her first since giving birth to her son Leo in December 2016.

Madison Keys was the defending champion, but lost in the second round to Ons Jabeur.

Seeds
The top eight seeds received a bye into the second round. • 🇨🇿 Karolína Plíšková (second round)

• 🇺🇸 Sofia Kenin (second round)

• 🇺🇸 Serena Williams (third round)

• 🇯🇵 Naomi Osaka (final, withdrew)

• 🇧🇾 Aryna Sabalenka (third round)

• 🇨🇿 Petra Kvitová (second round)

• 🇺🇸 Madison Keys (second round)

• 🇬🇧 Johanna Konta (semifinals)

• 🇰🇿 Elena Rybakina (first round)

• 🇨🇿 Markéta Vondroušová (first round)

• 🇺🇸 Alison Riske (first round)

• 🇪🇪 Anett Kontaveit (quarterfinals)

• Maria Sakkari (quarterfinals)

• 🇧🇪 Elise Mertens (semifinals)

• Donna Vekić (first round)

• 🇺🇦 Dayana Yastremska (third round)

Seeds
• # Jil Teichmann (qualified)

• # 🇷🇺 Anna Blinkova (first round)

• # 🇺🇸 Lauren Davis (first round)

• # 🇰🇿 Zarina Diyas (first round)

• # 🇩🇪 Laura Siegemund (qualified)

• # 🇷🇺 Daria Kasatkina (qualifying competition, lucky loser)

• # Arantxa Rus (qualified)

• # 🇯🇵 Nao Hibino (first round)

• # 🇺🇸 Taylor Townsend (first round)

• # 🇷🇴 Sorana Cîrstea (qualifying competition)

• # 🇯🇵 Misaki Doi (first round)

• # 🇧🇪 Kirsten Flipkens (qualified)

• # 🇺🇸 Madison Brengle (first round)

• # 🇺🇸 Jessica Pegula (qualified)

• # 🇸🇰 Viktória Kužmová (qualifying competition)

• # 🇷🇴 Patricia Maria Țig (first round)

• # 🇷🇸 Nina Stojanović (qualifying competition)

• # 🇺🇸 Christina McHale (qualified)

• # Monica Puig (first round)

• # 🇲🇪 Danka Kovinić (first round)

• # 🇪🇸 Paula Badosa (qualifying competition)

• # 🇺🇸 Shelby Rogers (qualifying competition)

• # 🇮🇹 Jasmine Paolini (first round)

• # 🇺🇸 Kristie Ahn (qualifying competition)

Qualifiers
• # Jil Teichmann

• # 🇨🇦 Leylah Fernandez

• # 🇺🇸 Jessica Pegula

• # 🇺🇸 Ann Li

• # 🇩🇪 Laura Siegemund

• # 🇺🇸 Christina McHale

• # Arantxa Rus

• # 🇺🇸 Catherine Bellis

• # 🇷🇺 Vera Zvonareva

• # 🇷🇺 Anna Kalinskaya

• # 🇫🇷 Océane Dodin

• # 🇧🇪 Kirsten Flipkens

Lucky loser

 * 1) 🇷🇺 Daria Kasatkina