2021 Astana Open – Women's singles

This was the first edition of the women's event.

Alison Van Uytvanck won the title, defeating Yulia Putintseva in the final, 1–6, 6–4, 6–3.

Seeds
• # 🇰🇿 Yulia Putintseva (final)

• # 🇧🇪 Alison Van Uytvanck (champion)

• # 🇫🇷 Kristina Mladenovic (second round)

• # 🇧🇪 Greet Minnen (first round)

• # Ana Konjuh (second round)

• # 🇸🇪 Rebecca Peterson (semifinals)

• # 🇷🇺 Varvara Gracheva (quarterfinals)

• # 🇫🇷 Clara Burel (second round)

Seeds
• # 🇮🇹 Lucia Bronzetti (qualifying competition)

• # 🇬🇧 Katie Boulter (qualified)

• # 🇺🇦 Lesia Tsurenko (qualified)

• # 🇷🇸 Aleksandra Krunić (qualified)

• # 🇷🇺 Natalia Vikhlyantseva (qualified)

• # 🇷🇺 Valeria Savinykh (first round)

• # 🇵🇱 Urszula Radwańska (qualifying competition, lucky loser)

• # 🇷🇴 Monica Niculescu (qualifying competition, withdrew)

• # 🇷🇺 Anastasia Zakharova (qualified)

• # 🇧🇾 Yuliya Hatouka (qualified)

• # 🇬🇧 Samantha Murray Sharan (qualifying competition)

• # 🇺🇦 Valeriya Strakhova (qualifying competition)

Qualifiers
• # 🇧🇾 Yuliya Hatouka

• # 🇬🇧 Katie Boulter

• # 🇺🇦 Lesia Tsurenko

• # 🇷🇸 Aleksandra Krunić

• # 🇷🇺 Natalia Vikhlyantseva

• # 🇷🇺 Anastasia Zakharova

Lucky loser
• # 🇵🇱 Urszula Radwańska