2021 Biella Challenger VII – Singles

Thanasi Kokkinakis was the defending champion but chose not to defend his title.

Holger Rune won the title after defeating Marco Trungelliti 6–3, 5–7, 7–6(7–5) in the final.

Seeds
• # 🇵🇪 Juan Pablo Varillas (second round)

• # 🇷🇸 Nikola Milojević (second round)

• # 🇩🇪 Daniel Altmaier (first round)

• # 🇷🇸 Danilo Petrović (first round, retired)

• # Frederico Ferreira Silva (second round)

• # 🇵🇱 Kacper Żuk (quarterfinals)

• # 🇸🇰 Martin Kližan (second round)

• # 🇮🇹 Lorenzo Giustino (second round)