2021 Challenger Ciudad de Guayaquil

The 2021 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the seventeenth edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 1 and 7 November 2021.

Seeds

 * 1 Rankings are as of 25 October 2021.

Other entrants
The following players received wildcards into the singles main draw:
 * 🇦🇷 Facundo Bagnis
 * 🇪🇨 Álvaro Guillén Meza
 * 🇪🇨 Diego Hidalgo

The following players received entry into the singles main draw as alternates:
 * 🇦🇷 Facundo Díaz Acosta
 * 🇨🇦 Alexis Galarneau

The following players received entry from the qualifying draw:
 * 🇵🇪 Nicolás Álvarez
 * 🇧🇷 Daniel Dutra da Silva
 * 🇨🇴 Alejandro González
 * 🇦🇷 Facundo Juárez

The following player received entry as a lucky loser:
 * 🇪🇸 Nicolás Álvarez Varona

Singles

 * Alejandro Tabilo def. Jesper de Jong 6–1, 7–5.

Doubles

 * Jesper de Jong / Bart Stevens def. 🇪🇨 Diego Hidalgo / 🇨🇴 Cristian Rodríguez 7–5, 6–2.