2021 Challenger Ciudad de Guayaquil – Singles

Francisco Cerúndolo was the defending champion but lost in the second round to Gonçalo Oliveira.

Alejandro Tabilo won the title after defeating Jesper de Jong 6–1, 7–5 in the final.

Seeds
• # 🇪🇸 Jaume Munar (first round)

• # 🇦🇷 Facundo Bagnis (quarterfinals)

• # 🇨🇴 Daniel Elahi Galán (quarterfinals)

• # 🇦🇷 Sebastián Báez (first round)

• # 🇦🇷 Francisco Cerúndolo (second round)

• # 🇧🇷 Thiago Seyboth Wild (semifinals)

• # 🇧🇴 Hugo Dellien (second round)

• # 🇦🇷 Tomás Martín Etcheverry (semifinals)