2021 Copa Colsanitas – Singles

Camila Osorio defeated Tamara Zidanšek in the final, 5–7, 6–3, 6–4 to win the singles tennis title at the 2021 Copa Colsanitas. It was the Colombian teenage wildcard's first WTA Tour singles title. Ranked No. 180, Osorio became the lowest-ranked WTA title holder since world No. 299 Margarita Gasparyan at the 2018 Tashkent Open. Zidanšek was also in contention for her first WTA title.

Amanda Anisimova was the reigning champion from when the tournament was last held in 2019, but chose to compete in Charleston instead.

Seeds
• # 🇨🇳 Zheng Saisai (second round)

• # 🇪🇸 Sara Sorribes Tormo (first round)

• # Arantxa Rus (second round)

• # Clara Tauson (first round)

• # Tamara Zidanšek (final)

• # 🇮🇹 Jasmine Paolini (second round)

• # 🇨🇿 Tereza Martincová (second round)

• # 🇨🇳 Wang Yafan (second round)

Seeds
• # 🇦🇺 Arina Rodionova (first round)

• # 🇪🇸 Lara Arruabarrena (qualified)

• # 🇮🇹 Giulia Gatto-Monticone (qualified)

• # 🇪🇸 Nuria Párrizas Díaz (qualified)

• # 🇫🇷 Clara Burel (first round)

• # 🇫🇷 Chloé Paquet (qualified)

• # 🇫🇷 Harmony Tan (qualified)

• # Mariam Bolkvadze (first round)

• # 🇺🇸 Robin Anderson (qualifying competition)

• # 🇷🇸 Natalija Kostić (first round)

• # 🇺🇸 Danielle Lao (first round)

• # 🇷🇺 Marina Melnikova (qualifying competition)

Qualifiers
• # 🇫🇷 Harmony Tan

• # 🇪🇸 Lara Arruabarrena

• # 🇮🇹 Giulia Gatto-Monticone

• # 🇪🇸 Nuria Párrizas Díaz

• # Daniela Seguel

• # 🇫🇷 Chloé Paquet