2021 Eastbourne International – Women's singles

Jeļena Ostapenko defeated Anett Kontaveit in the final, 6–3, 6–3, to win the women's singles tennis title at the 2021 Eastbourne International. By winning her fourth career WTA Tour singles title, Ostapenko became the tournament's third wildcard champion, following Monica Seles (1996) and Julie Halard-Decugis (2000).

Karolína Plíšková was the defending champion from when the event was last held in 2019, but she lost in the first round to Camila Giorgi. As a result, Plíšková exited the top 10 of the WTA rankings for the first time since September 2016.

Seeds
• #🇧🇾 Aryna Sabalenka (quarterfinals)

• #🇺🇦 Elina Svitolina (second round)

• #🇨🇦 Bianca Andreescu (second round)

• #🇵🇱 Iga Świątek (second round)

• #🇨🇿 Karolína Plíšková (first round)

• # Belinda Bencic (second round)

• #🇧🇪 Elise Mertens (first round)

• #🇷🇺 Anastasia Pavlyuchenkova (first round)

Seeds
• # 🇨🇳 Zhang Shuai (qualifying competition)

• # 🇰🇿 Yulia Putintseva (first round)

• # 🇵🇱 Magda Linette (first round)

• # 🇺🇸 Shelby Rogers (qualifying competition, lucky loser)

• # 🇫🇷 Fiona Ferro (first round)

• # Anastasija Sevastova (qualifying competition, lucky loser)

• # 🇫🇷 Kristina Mladenovic (qualifying competition)

• # Hsieh Su-wei (withdrew, still competing in Birmingham)

• # 🇺🇦 Marta Kostyuk (qualified)

• # 🇺🇸 Bernarda Pera (qualified)

• # Viktorija Golubic (qualified)

• # 🇦🇺 Ajla Tomljanović (qualifying competition)

Qualifiers
• # 🇺🇦 Marta Kostyuk

• # Viktorija Golubic

• # 🇮🇹 Camila Giorgi

• # 🇺🇸 Bernarda Pera

• # 🇷🇺 Vera Zvonareva

• # 🇺🇸 Christina McHale

Lucky losers
• # 🇺🇸 Shelby Rogers

• # Anastasija Sevastova