2021 I.ČLTK Prague Open

The 2021 I.ČLTK Prague Open was a professional tennis tournament played on outdoor clay courts. It was the 28th (men) and 16th (women) editions of the tournament which was part of the 2021 ATP Challenger Tour and the 2021 ITF Women's World Tennis Tour. It took place in Prague, Czech Republic from 3 to 9 May 2021.

Seeds

 * 1 Rankings are as of 26 April 2021.

Other entrants
The following players received wildcards into the singles main draw:
 * 🇨🇿 Jiří Lehečka
 * 🇨🇿 Andrew Paulson
 * 🇨🇿 Michael Vrbenský

The following player received entry into the singles main draw as a special exempt:
 * 🇦🇷 Renzo Olivo

The following players received entry from the qualifying draw:
 * 🇨🇿 Zdeněk Kolář
 * 🇨🇿 Vít Kopřiva
 * 🇺🇦 Sergiy Stakhovsky
 * 🇵🇱 Kacper Żuk

Seeds

 * 1 Rankings are as of 26 April 2021.

Other entrants
The following players received wildcards into the singles main draw:
 * 🇨🇿 Nikola Bartůňková
 * Ana Konjuh
 * 🇨🇿 Linda Nosková
 * 🇨🇿 Darja Viďmanová

The following player received entry using a protected ranking:
 * 🇮🇳 Karman Thandi

The following players received entry from the qualifying draw:
 * 🇭🇺 Anna Bondár
 * 🇬🇧 Amanda Carreras
 * 🇨🇿 Johana Marková
 * 🇩🇪 Jule Niemeier
 * 🇦🇷 Paula Ormaechea
 * 🇹🇷 İpek Öz
 * 🇸🇰 Chantal Škamlová
 * 🇧🇾 Shalimar Talbi

Men's singles

 * Tallon Griekspoor def. 🇩🇪 Oscar Otte 5–7, 6–4, 6–4.

Women's singles

 * 🇩🇪 Jule Niemeier def. 🇭🇺 Dalma Gálfi, 6–4, 6–2

Men's doubles

 * 🇦🇺 Marc Polmans / 🇺🇦 Sergiy Stakhovsky def. Ivan Sabanov /  Matej Sabanov 6–3, 6–4.

Women's doubles

 * 🇭🇺 Anna Bondár / 🇧🇪 Kimberley Zimmermann def. Xenia Knoll / 🇷🇴 Elena-Gabriela Ruse, 7–6(7–5), 6–2