2021 I.ČLTK Prague Open – Women's singles

The women's singles of the 2021 I.ČLTK Prague Open tournament was played on clay in Prague, Czech Republic.

Tamara Korpatsch was the defending champion, but chose not to participate.

Jule Niemeier won the title, starting from qualifications, defeating Dalma Gálfi in the final, 6–4, 6–2.

Seeds
• # 🇸🇰 Viktória Kužmová (second round)

• # 🇷🇴 Jaqueline Cristian (first round)

• # 🇦🇹 Barbara Haas (second round)

• # 🇷🇴 Elena-Gabriela Ruse (semifinals)

• # 🇹🇷 Çağla Büyükakçay (quarterfinals)

• # Lesley Pattinama Kerkhove (first round)

• # 🇸🇰 Rebecca Šramková (second round)

• # 🇦🇹 Julia Grabher (second round)