2021 IBG Prague Open – Singles

Aslan Karatsev was the defending champion but chose not to defend his title.

Franco Agamenone won the title after defeating Ryan Peniston 6–3, 6–1 in the final.

Seeds
• # 🇷🇺 Evgeny Karlovskiy (quarterfinals)

• # 🇺🇸 Alexander Ritschard (second round)

• # 🇷🇺 Evgenii Tiurnev (first round)

• # 🇫🇷 Geoffrey Blancaneaux (second round)

• # 🇨🇿 Jonáš Forejtek (first round)

• # 🇧🇪 Michael Geerts (quarterfinals)

• # 🇷🇴 Filip Jianu (first round)

• # 🇷🇸 Miljan Zekić (first round, retired)