2021 NCAA Division I softball tournament

The 2021 NCAA Division I softball tournament was held from May 21 through June 10, 2021 as the final part of the 2021 NCAA Division I softball season. 31 teams were awarded automatic bids as champions of their conferences after the Ivy League opted out of the 2021 softball season. The remaining 33 were selected at-large by the NCAA Division I softball selection committee on May 16, 2021. The tournament culminated with eight teams playing in the 2021 Women's College World Series at USA Softball Hall of Fame Stadium in Oklahoma City.

Automatic bids
The Big Ten, Big West, Mountain West, Pac-12, and West Coast Conference bids were awarded to the regular-season champion. All other conferences had their automatic bid go to the conference tournament winner.

National seeds
16 National Seeds were announced on the Selection Show, on Sunday, May 16 at 9 p.m. EDT on ESPN2. 15 of the 16 national seeds hosted Regionals. Teams in italics advanced to Super Regionals. Teams in bold advanced to the Women's College World Series.

1. Oklahoma 2.  3. Alabama 4. ' 5. ' 6. Arkansas'' 7. ' 8. '

9. 10. ' 11. ' 12. Texas 13. (not a host) 14. ' 15. 16. '

Regionals and Super Regionals
The Regionals took place May 20–23. The Super Regionals took place May 27–30.

Women's College World Series
The Women's College World Series was held June 3 through June 10 in Oklahoma City.

Participants
† = From NCAA Division I Softball Championship Results

All-tournament Team
The following players were members of the Women's College World Series All-Tournament Team.

Record by conference
The columns RF, SR, WS, NS, F, and NC respectively stand for the Regional Finals, Super Regionals, College World Series Teams, National Semi-Finals, Finals, and National Champion.

Radio
For the first time ever Westwood One will provide nationwide radio coverage of every game in the Women's College World Series. It was streamed online at westwoodsports.com, through TuneIn, and on SiriusXM. Ryan Radtke and Leah Amico return as the broadcast team.

Television
ESPN held exclusive rights to the tournament. The network aired games across ESPN, ESPN2, ESPNU, SEC Network, Longhorn Network, ACC Network and ESPN3. For just the fourth time in the history of the women's softball tournament, ESPN covered every regional. ABC will air a super regional game for the first time in tournament history.

Broadcast assignments
Regionals Super Regionals Women's College World Series
 * Norman: Courtney Lyle & Kayla Braud
 * Los Angeles: Mark Neely & Kenzie Fowler
 * Tuscaloosa: Eric Frede & Madison Shipman
 * Gainesville: Beth Mowins & Michele Smith
 * Stillwater: Clay Matvick & Natasha Watley
 * Fayetteville: Mike Corey & Leah Amico
 * Baton Rouge: Alex Loeb & Megan Willis
 * Columbia: Tyler Denning & Tori Vidales
 * Norman: Beth Mowins, Jessica Mendoza, Michele Smith & Holly Rowe
 * Columbia: Mike Couzens & Carol Bruggeman
 * Stillwater: Tiffany Greene & Erin Miller
 * Gainesville: Courtney Lyle & Kayla Braud
 * Kevin Brown, Amanda Scarborough & Jalyn Johnson (afternoons, early Fri)
 * Beth Mowins, Jessica Mendoza, Michele Smith & Holly Rowe (evenings minus early Fri)

Regionals Super Regionals Women's College World Series Finals
 * Knoxville: Tiffany Greene & Erin Miller
 * Tallahassee: Jenn Hildreth & Brittany McKinney
 * Tucson: Jonathan Yardley & Amanda Freed
 * Austin: Kevin Brown, Amanda Scarborough & Jalyn Johnson
 * Athens: Alex Perlman & Francesca Enea
 * Lexington: Mike Couzens & Carol Bruggeman
 * Tempe: John Schriffen & Jennie Ritter
 * Seattle: Pam Ward & Jenny Dalton-Hill
 * Tuscaloosa: Eric Frede & Madison Shipman
 * Fayetteville: Pam Ward & Jenny Dalton Hill
 * Baton Rouge: Kevin Brown, Amanda Scarborough & Jalyn Johnson
 * Los Angeles: Mark Neely & Kenzie Fowler
 * Beth Mowins, Jessica Mendoza, Michele Smith & Holly Rowe