2021 Nicholls Colonels football team

The 2021 Nicholls State Colonels football team represented Nicholls State University as a member of the Southland Conference during the 2021 NCAA Division I FCS football season. Led by seventh-year head coach Tim Rebowe, the Colonels compiled an overall record of 6–5 with a mark of 5–3 in conference play, placing third in the Southland. Nicholls State played home games at John L. Guidry Stadium in Thibodaux, Louisiana.

Preseason poll
The Southland Conference released their preseason poll in July 2021. The Colonels were picked to finish second in the conference. In addition, eleven Colonels were chosen to the Preseason All-Southland Team.

Preseason All–Southland Teams
Offense

1st Team
 * David Mosley – Tight End/Halfback, SR
 * Dai'Jean Dixon – Wide Receiver, SR
 * P. J. Burkhalter – Offensive Lineman, SR
 * Jair Joseph – Offensive Lineman, SR
 * Evan Roussel – Offensive Lineman, SO

2nd Team
 * Julien Gums – Running Back, SR
 * KJ Franklin – Wide Receiver, JR

Defense

1st Team
 * Kevin Moore III – Defensive Back, SR

2nd Team
 * Perry Ganci – Defensive Lineman, SO
 * Hayden Shaheen – Linebacker, FR
 * Dontaze Costly – Kick Returner, SR