2021 Open Città di Bari – Singles

This was the first edition of the tournament.

Oscar Otte won the title after defeating Daniel Masur 7–5, 7–5 in the final.

Seeds
• # 🇪🇸 Carlos Taberner (second round)

• # Holger Rune (first round, retired)

• # 🇩🇪 Oscar Otte (champion)

• # 🇺🇸 Maxime Cressy (first round)

• # 🇷🇸 Nikola Milojević (first round)

• # 🇮🇹 Federico Gaio (first round)

• # 🇮🇹 Roberto Marcora (first round)

• # 🇩🇪 Daniel Masur (final)