2021 Prague Open – Singles

Simona Halep was the defending champion, but chose not to participate.

Barbora Krejčíková won the title, defeating Tereza Martincová in the final, 6–2, 6–0.

Seeds
• # 🇨🇿 Petra Kvitová (first round)

• # 🇨🇿 Barbora Krejčíková (champion)

• # 🇨🇿 Markéta Vondroušová (withdrew)

• # 🇨🇿 Marie Bouzková (first round)

• # 🇨🇿 Kateřina Siniaková (quarterfinals)

• # Hsieh Su-wei (withdrew)

• # 🇷🇸 Nina Stojanović (second round)

• # 🇨🇿 Tereza Martincová (final)

• # 🇧🇪 Greet Minnen (semifinals)

Seeds
• # 🇷🇺 Anastasia Gasanova (qualifying competition; Lucky loser)

• # 🇺🇸 Asia Muhammad (qualified)

• # 🇧🇬 Isabella Shinikova (qualified)

• # 🇬🇧 Samantha Murray Sharan (moved to the main draw)

• # 🇸🇰 Rebecca Šramková (qualified)

• # 🇵🇱 Urszula Radwańska (qualified)

• # Conny Perrin (qualifying competition; Lucky loser)

• # Liang En-shuo (qualifying competition; Lucky loser)

• # 🇷🇺 Anastasia Zakharova (qualifying competition)

• # 🇬🇧 Jodie Burrage (qualified)

• # 🇨🇳 Yuan Yue (qualifying competition)

• # 🇬🇧 Naiktha Bains (qualified)

Qualifiers
• # 🇬🇧 Jodie Burrage

• # 🇺🇸 Asia Muhammad

• # 🇧🇬 Isabella Shinikova

• # 🇬🇧 Naiktha Bains

• # 🇸🇰 Rebecca Šramková

• # 🇵🇱 Urszula Radwańska

Lucky losers
• # Conny Perrin

• # 🇷🇺 Anastasia Gasanova

• # Liang En-shuo