2021 São Paulo Challenger de Tênis – Singles

Felipe Meligeni Alves was the defending champion but withdrew before his semifinal match with Luciano Darderi.

Juan Pablo Ficovich won the title after defeating Darderi 6–3, 7–5 in the final.

Seeds
• # Pablo Cuevas (withdrew)

• # 🇧🇴 Hugo Dellien (quarterfinals)

• # 🇧🇷 Thiago Seyboth Wild (second round)

• # 🇦🇷 Juan Ignacio Londero (first round)

• # Nicolás Jarry (semifinals)

• # 🇧🇷 Felipe Meligeni Alves (semifinals, withdrew)

• # 🇦🇷 Nicolás Kicker (first round)

• # 🇦🇷 Pedro Cachin (second round)