2021 Serbia Open – Women's singles

This was the first edition of the tournament.

Paula Badosa won her first WTA singles title without dropping a set throughout the entire tournament, after Ana Konjuh retired from the final with the scoreline at 6–2, 2–0.

This was Konjuh’s first tour-level final since 2017 ASB Classic tournament following her comeback from injury.

Seeds
• #🇷🇺 Anastasia Pavlyuchenkova (withdrew)

• #🇰🇿 Yulia Putintseva (second round)

• #🇨🇳 Zhang Shuai (first round)

• #🇪🇸 Paula Badosa (champion)

• #🇦🇷 Nadia Podoroska (quarterfinals)

• #🇫🇷 Kristina Mladenovic (first round)

• #🇸🇪 Rebecca Peterson (quarterfinals)

• #🇲🇪 Danka Kovinić (first round)

Seeds
• # 🇨🇴 Camila Osorio (qualified)

• # 🇧🇬 Viktoriya Tomova (qualifying competition, lucky loser)

• # 🇨🇳 Wang Xiyu (qualified)

• # 🇷🇺 Kamilla Rakhimova (qualified)

• # 🇷🇴 Jaqueline Cristian (qualifying competition)

• # 🇪🇸 Nuria Párrizas Díaz (first round)

• # 🇪🇸 Cristina Bucșa (qualified)

• # 🇷🇺 Anastasia Gasanova (qualifying competition)

• # 🇨🇳 You Xiaodi (qualifying competition)

• # Ana Konjuh (qualified)

• # Daniela Seguel (qualifying competition)

• # 🇷🇸 Aleksandra Krunić (first round)

Qualifiers
• # 🇨🇴 Camila Osorio

• # Ana Konjuh

• # 🇨🇳 Wang Xiyu

• # 🇷🇺 Kamilla Rakhimova

• # 🇭🇺 Réka Luca Jani

• # 🇪🇸 Cristina Bucșa

Lucky loser
• # 🇧🇬 Viktoriya Tomova