2021 TK Sparta Prague Open – Singles

Adam Pavlásek was the defending champion but chose not to defend his title.

Dalibor Svrčina won the title after defeating Dmitry Popko 6–0, 7–5 in the final.

Seeds
• # 🇷🇸 Nikola Milojević (quarterfinals)

• # 🇰🇿 Dmitry Popko (final)

• # 🇮🇹 Lorenzo Giustino (second round)

• # 🇦🇷 Facundo Mena (semifinals)

• # Nicolás Jarry (first round)

• # 🇮🇹 Riccardo Bonadio (quarterfinals)

• # 🇪🇸 Adrián Menéndez Maceiras (second round)

• # 🇨🇿 Michael Vrbenský (first round)