2021 Tallahassee Tennis Challenger – Singles

Emilio Gómez was the defending champion but chose not to defend his title.

Jenson Brooksby won the title after defeating Bjorn Fratangelo 6–3, 4–6, 6–3 in the final.

Seeds
• # 🇧🇷 Thiago Seyboth Wild (second round)

• # 🇺🇸 Denis Kudla (semifinals)

• # 🇮🇳 Prajnesh Gunneswaran (first round)

• # Jason Jung (second round)

• # Ivo Karlović (first round)

• # 🇦🇷 Juan Manuel Cerúndolo (first round)

• # 🇰🇿 Dmitry Popko (first round)

• # 🇺🇸 Michael Mmoh (quarterfinals, retired)