2021 US Open – Women's singles qualifying

The 2021 US Open – Women's Singles Qualifying was a series of tennis matches from 24 August 2021 to 27 August 2021 where 128 players competed to determine the 16 qualifiers into the main draw of the 2021 US Open women's singles tournament, and, if necessary, the lucky losers. Due to pandemic-related restrictions, the qualifying matches were not open to the public.

For the first time in Major singles history, a qualifier, Emma Raducanu, went on to win the main tournament.

Seeds
• # Ana Konjuh (qualified)

• #🇸🇰 Anna Karolína Schmiedlová (qualified)

• #🇪🇸 Nuria Párrizas Díaz (qualified)

• #🇪🇬 Mayar Sherif (qualifying competition, lucky loser)

• #🇫🇷 Océane Dodin (qualifying competition)

• #🇧🇪 Greet Minnen (qualifying competition, lucky loser)

• #🇷🇴 Elena-Gabriela Ruse (qualified)

• #🇸🇰 Kristína Kučová (qualifying competition, lucky loser)

• #🇧🇪 Maryna Zanevska (second round)

• #🇦🇺 Astra Sharma (qualified)

• #🇨🇿 Kristýna Plíšková (qualified)

• #🇧🇬 Viktoriya Tomova (qualifying competition, lucky loser)

• #🇷🇴 Irina Bara (first round)

• #🇲🇽 Renata Zarazúa (first round)

• #🇵🇱 Magdalena Fręch (first round)

• #🇩🇪 Anna-Lena Friedsam (second round)

• #🇺🇦 Kateryna Kozlova (first round)

• # Stefanie Vögele (qualifying competition, lucky loser)

• #🇧🇪 Ysaline Bonaventure (second round)

• #🇷🇺 Kamilla Rakhimova (qualifying competition, lucky loser)

• #🇧🇾 Olga Govortsova (first round)

• #🇷🇺 Anna Kalinskaya (second round)

• #🇫🇷 Harmony Tan (qualifying competition)

• #🇷🇺 Vitalia Diatchenko (second round)

• #🇺🇸 Kristie Ahn (first round)

• #🇷🇴 Jaqueline Cristian (qualifying competition)

• #🇺🇦 Katarina Zavatska (first round)

• #🇦🇺 Maddison Inglis (first round)

• #🇷🇸 Olga Danilović (qualified)

• #🇩🇪 Jule Niemeier (second round)

• #🇬🇧 Emma Raducanu (qualified, main draw champion)

• #🇬🇧 Harriet Dart (qualified)

Qualifiers
• # Ana Konjuh

• #🇸🇰 Anna Karolína Schmiedlová

• #🇪🇸 Nuria Párrizas Díaz

• #🇬🇧 Emma Raducanu

• #🇪🇸 Cristina Bucșa

• #🇨🇦 Rebecca Marino

• #🇷🇴 Elena-Gabriela Ruse

• #🇬🇧 Katie Boulter

• # Valentini Grammatikopoulou

• #🇦🇺 Astra Sharma

• #🇨🇿 Kristýna Plíšková

• #🇬🇧 Harriet Dart

• #🇷🇸 Olga Danilović

• #🇭🇺 Dalma Gálfi

• #🇪🇸 Rebeka Masarova

• #🇺🇸 Jamie Loeb

Lucky losers
• #🇪🇬 Mayar Sherif

• #🇸🇰 Kristína Kučová

• #🇧🇬 Viktoriya Tomova

• #🇧🇪 Greet Minnen

• # Stefanie Vögele

• #🇷🇺 Kamilla Rakhimova