2022 Challenger del Biobío – Singles

This was the first edition of the tournament.

Tomás Martín Etcheverry won the title after defeating Hugo Dellien 6–3, 6–2 in the final.

Seeds
• # 🇧🇴 Hugo Dellien (final)

• # 🇵🇪 Juan Pablo Varillas (quarterfinals)

• # 🇦🇷 Tomás Martín Etcheverry (champion)

• # Nicolás Jarry (second round)

• # Tomás Barrios Vera (semifinals)

• # 🇨🇿 Vít Kopřiva (first round)

• # 🇦🇷 Camilo Ugo Carabelli (quarterfinals)

• # 🇦🇷 Facundo Mena (second round)