2022 I.ČLTK Prague Open – Women's singles

Jule Niemeier was the defending champion but chose not to participate.

Maja Chwalińska won the title, defeating Ekaterine Gorgodze in the final, 7–5, 6–3.

Seeds
• # Ekaterine Gorgodze (final)

• # 🇭🇺 Réka Luca Jani (quarterfinals)

• # 🇨🇳 Yuan Yue (first round)

• # Despina Papamichail (first round)

• # 🇸🇰 Rebecca Šramková (first round)

• # Valentini Grammatikopoulou (first round)

• # Suzan Lamens (withdrew)

• # 🇦🇺 Lizette Cabrera (quarterfinals)