2023 Advantage Cars Prague Open – Women's singles

Maja Chwalińska was the defending champion but lost to Anastasia Kulikova in the first round.

Darja Semeņistaja won the title, defeating Jéssica Bouzas Maneiro in the final, 2–6, 6–3, 6–4.

Seeds
• # 🇭🇺 Réka Luca Jani (second round)

• # 🇦🇺 Priscilla Hon (quarterfinals)

• # 🇮🇹 Nuria Brancaccio (second round)

• # 🇪🇸 Jéssica Bouzas Maneiro (final)

• # 🇲🇽 Fernanda Contreras (second round)

• # 🇨🇿 Barbora Palicová (first round)

• # Céline Naef (second round)

• # Darja Semeņistaja (champion)