2023 Challenger Ciudad de Guayaquil – Singles

Daniel Altmaier was the defending champion but chose not to defend his title.

Alejandro Tabilo won the title after defeating Daniel Elahi Galán 6–2, 6–2 in the final.

Seeds
• # 🇦🇷 Federico Coria (quarterfinals)

• # 🇨🇴 Daniel Elahi Galán (final)

• # 🇦🇷 Facundo Díaz Acosta (quarterfinals, retired)

• # 🇦🇷 Juan Manuel Cerúndolo (first round)

• # Alejandro Tabilo (champion)

• # 🇧🇴 Hugo Dellien (quarterfinals)

• # 🇧🇷 Felipe Meligeni Alves (semifinals)

• # 🇦🇷 Camilo Ugo Carabelli (quarterfinals)