2023 Charlottesville Men's Pro Challenger – Singles

Ben Shelton was the defending champion but chose not to defend his title.

Beibit Zhukayev won the title after defeating Aidan Mayo 6–3, 6–4 in the final.

Seeds
• # 🇺🇸 Michael Mmoh (second round)

• # 🇺🇸 Alex Michelsen (first round)

• # 🇫🇷 Benoît Paire (quarterfinals)

• # 🇺🇸 Zachary Svajda (first round)

• # 🇺🇸 Emilio Nava (second round)

• # 🇦🇺 Adam Walton (first round)

• # 🇺🇸 Denis Kudla (second round)

• # 🇺🇸 Tennys Sandgren (first round)