2023 Italian Open – Women's singles

Elena Rybakina won the women's singles tennis title at the 2023 Italian Open after Anhelina Kalinina retired from the final with the scoreline at 6–4, 1–0. It was Rybakina's second career WTA 1000 title. Rybakina won three of her six matches at the tournament via retirement: over Anna Kalinskaya in the third round, Iga Świątek in the quarterfinals, and Kalinina in the final. With the win, Rybakina entered the top five in the WTA rankings for the first time at world No. 4.

Świątek was the two-time defending champion, but retired in the quarterfinals against Rybakina due to a hip injury. Świątek was attempting to be the first woman to win three consecutive titles in Rome since Conchita Martínez in 1995.

Seeds
All seeds received a bye into the second round. • 🇵🇱 Iga Świątek (quarterfinals, retired)

• Aryna Sabalenka (second round)

• 🇺🇸 Jessica Pegula (second round)

• 🇹🇳 Ons Jabeur (second round)

• 🇫🇷 Caroline Garcia (third round)

• 🇺🇸 Coco Gauff (third round)

• 🇰🇿 Elena Rybakina (champion)

•  Daria Kasatkina (fourth round)

•  Maria Sakkari (third round)

• 🇨🇿 Barbora Krejčíková (third round)

•  Veronika Kudermetova (semifinals)

• 🇧🇷 Beatriz Haddad Maia (quarterfinals)

• 🇨🇿 Karolína Plíšková (second round)

•  Victoria Azarenka (third round, withdrew)

•  Ekaterina Alexandrova (second round)

•  Liudmila Samsonova (third round)

• 🇵🇱 Magda Linette (third round)

• 🇮🇹 Martina Trevisan (second round)

• 🇺🇸 Madison Keys (fourth round)

•  Jeļena Ostapenko (semifinals)

•  Donna Vekić (fourth round)

• 🇨🇳 Zheng Qinwen (quarterfinals)

•  Anastasia Potapova (third round)

• 🇨🇦 Bianca Andreescu (second round)

• 🇧🇪 Elise Mertens (second round)

•  Jil Teichmann (second round)

• 🇨🇿 Marie Bouzková (fourth round)

• 🇺🇸 Bernarda Pera (second round)

•  Petra Martić (second round)

• 🇺🇦 Anhelina Kalinina (final, retired)

• 🇷🇴 Irina-Camelia Begu (second round)

• 🇺🇦 Marta Kostyuk (third round)

Seeded players
The following are the seeded players. Seedings are based on WTA rankings as of 24 April 2023. Rankings and points before are as of 8 May 2023.

Because the women's tournament is being expanded to two weeks this year, players are defending points from the 2022 Italian Open, as well as tournaments that took place during the week of 16 May 2022 (Rabat and Strasbourg). Points from the 2022 Italian Open are listed first in the "Points defending" column.

The event is not mandatory on the women's side and points from the 2022 Italian Open are included in the table below only if they counted towards the player's ranking as of 8 May 2023. For other players, the points defending column shows the lower of (a) points from the player's second-highest non-mandatory WTA 1000 tournament (which are required to be counted in her ranking) or (b) her 16th best result.

Points defending will be replaced at the end of the tournament by the highest of (a) the player's points from the 2023 tournament, (b) her 17th best result, or (c) points from her second-highest non-mandatory WTA 1000 event.

† Points from the player's 16th best result (for points defending) or 17th best result (for points earned), in each case as of 8 May 2023. ‡ Points from the player's second-best non-mandatory WTA 1000 event, which are required to be counted in her ranking. § The player is only defending points from Rabat or Strasbourg. ^ The player is defending points from Karlsruhe (WTA 125 tournament) and Strasbourg. & No change in points because points from this tournament did not count as one of the player's 16 best results.

Withdrawn players
The following players would have been seeded, but withdrew before the tournament began. † Points dropped were replaced with points from the player's next best result.

Wildcards
• 🇮🇹 Nuria Brancaccio

• 🇮🇹 Diletta Cherubini

• 🇮🇹 Sara Errani

• 🇮🇹 Matilde Paoletti

• 🇮🇹 Lisa Pigato

• 🇮🇹 Camilla Rosatello

• 🇮🇹 Dalila Spiteri

• 🇮🇹 Lucrezia Stefanini

Protected ranking
• 🇺🇸 Sofia Kenin

• Anastasia Pavlyuchenkova

• 🇦🇷 Nadia Podoroska

• 🇨🇿 Barbora Strýcová

• 🇺🇦 Elina Svitolina

• 🇨🇿 Markéta Vondroušová

Withdrawals
• & 🇺🇸 Amanda Anisimova → replaced by 🇧🇪 Maryna Zanevska

• & Belinda Bencic → replaced by 🇩🇪 Tatjana Maria

• & 🇺🇸 Danielle Collins → replaced by 🇺🇦 Lesia Tsurenko

• † 🇷🇴 Simona Halep → replaced by 🇪🇪 Anett Kontaveit

• & 🇨🇿 Petra Kvitová → replaced by 🇮🇹 Lucia Bronzetti

• & 🇬🇧 Emma Raducanu → replaced by 🇲🇪 Danka Kovinić

• & 🇨🇿 Kateřina Siniaková → replaced by 🇪🇸 Cristina Bucșa

• † 🇦🇺 Ajla Tomljanović → replaced by 🇫🇷 Alizé Cornet

• & 🇷🇴 Patricia Maria Țig → replaced by 🇪🇸 Nuria Párrizas Díaz

• & 🇨🇳 Zhang Shuai → replaced by 🇺🇸 Katie Volynets

• & 🇨🇳 Zhu Lin → replaced by  Kamilla Rakhimova

† – not included on entry list & – withdrew from entry list

Seeds
• # 🇧🇬 Viktoriya Tomova (qualified)

• # 🇪🇸 Rebeka Masarova (qualifying competition)

• # 🇧🇪 Ysaline Bonaventure (qualified)

• # 🇩🇪 Anna-Lena Friedsam (qualified)

• # 🇭🇺 Anna Bondár (qualified)

• # Kamilla Rakhimova (qualifying competition, lucky loser)

• # 🇭🇺 Dalma Gálfi (qualifying competition)

• # 🇨🇿 Tereza Martincová (qualified)

• # 🇫🇷 Océane Dodin (qualifying competition)

• # 🇵🇱 Magdalena Fręch (qualified)

• # Arantxa Rus (qualified)

• # 🇯🇵 Nao Hibino (qualified)

• # 🇦🇺 Kimberly Birrell (first round)

• # Erika Andreeva (first round)

• # 🇨🇴 Camila Osorio (qualified)

• # 🇪🇸 Marina Bassols Ribera (qualifying competition)

• # 🇺🇸 Taylor Townsend (qualified)

• # 🇧🇷 Laura Pigossi (first round)

• # 🇪🇸 Aliona Bolsova (qualifying competition)

• # Simona Waltert (qualifying competition)

• # 🇷🇸 Olga Danilović (first round)

• # 🇫🇷 Léolia Jeanjean (first round, retired)

• # 🇨🇦 Katherine Sebov (first round)

• # 🇬🇧 Harriet Dart (first round)

Qualifiers
• # 🇧🇬 Viktoriya Tomova

• # 🇺🇦 Dayana Yastremska

• # 🇧🇪 Ysaline Bonaventure

• # 🇩🇪 Anna-Lena Friedsam

• # 🇭🇺 Anna Bondár

• # 🇷🇴 Elena-Gabriela Ruse

• # 🇺🇸 Taylor Townsend

• # 🇨🇿 Tereza Martincová

• # 🇨🇴 Camila Osorio

• # 🇵🇱 Magdalena Fręch

• # Arantxa Rus

• # 🇯🇵 Nao Hibino

Lucky loser
• # Kamilla Rakhimova