2023 Lexington Challenger – Women's singles

Katie Swan was the defending champion but lost in the second round to Makenna Jones.

Renata Zarazúa won the title, defeating Caroline Dolehide in the final, 1–6, 7–6(7–4), 7–5.

Seeds
• # 🇺🇸 Caroline Dolehide (final)

• # 🇺🇸 Madison Brengle (second round)

• # 🇦🇺 Kimberly Birrell (quarterfinals)

• # 🇺🇸 Katie Volynets (second round)

• # 🇦🇺 Olivia Gadecki (semifinals)

• # 🇬🇧 Katie Swan (second round)

• # 🇯🇵 Mai Hontama (first round)

• # 🇨🇳 Wang Yafan (semifinals)