2023 Open delle Puglie – Singles

Tamara Zidanšek won the title, defeating Rebecca Šramková 3–6, 7–5, 6–1 in the final. Zidanšek saved four match points in the second set.

Julia Grabher was the reigning champion, but did not participate this year.

Seeds
• # 🇫🇷 Alizé Cornet (semifinals)

• # 🇷🇴 Jaqueline Cristian (quarterfinals)

• # 🇪🇸 Aliona Bolsova (second round)

• # 🇮🇹 Sara Errani (first round)

• # Tamara Zidanšek (champion)

• # 🇧🇷 Laura Pigossi (first round)

• # 🇪🇸 Marina Bassols Ribera (first round)

• # 🇩🇪 Noma Noha Akugue (first round)

Seeds
• # Jana Fett (moved to main draw)

• # Dalila Jakupović (moved to main draw)

• # 🇵🇱 Katarzyna Kawa (qualified)

• # 🇨🇿 Anna Sisková (qualifying competition)

Qualifiers
• # 🇬🇧 Freya Christie

• # 🇮🇹 Angelica Moratelli

• # 🇵🇱 Katarzyna Kawa

• # 🇰🇿 Zhibek Kulambayeva