2024 Advantage Cars Prague Open – Women's singles

Darja Semeņistaja was the defending champion but chose to compete in Rome instead.

Dominika Šalková won the title, defeating Maja Chwalińska in the final, 6–3, 6–0.

Seeds
• # Suzan Lamens (first round)

• # 🇭🇺 Panna Udvardy (second round)

• # 🇩🇪 Ella Seidel (second round)

• # 🇨🇿 Dominika Šalková (champion)

• # 🇺🇦 Kateryna Baindl (quarterfinals)

• # 🇩🇪 Noma Noha Akugue (second round)

• # 🇦🇺 Priscilla Hon (second round)

• # 🇹🇷 İpek Öz (semifinals)