2024 Fifth Third Charleston 125 – Singles

Varvara Lepchenko was the reigning champion from when the event was last held in 2021, but did not participate this year.

Elisabetta Cocciaretto won the title, defeating Diana Shnaider in the final, 6–3, 6–2.

Seeds
• #🇫🇷 Clara Burel (withdrew)

• # Arantxa Rus (first round)

• #🇮🇹 Martina Trevisan (second round)

• #🇮🇹 Elisabetta Cocciaretto (champion)

• #🇨🇳 Zhu Lin (first round)

• #🇨🇳 Wang Yafan (semifinals)

• # Diana Shnaider (final)

• #🇩🇪 Tamara Korpatsch (first round)

• #🇯🇵 Nao Hibino (second round)

Seeds
• # 🇺🇸 Emina Bektas (moved to main draw)

• # 🇪🇸 Rebeka Masarova (moved to main draw)

• # Aliaksandra Sasnovich (moved to main draw)

• # Erika Andreeva (qualified)

• # 🇮🇹 Sara Errani (moved to main draw)

• # 🇲🇽 Renata Zarazúa (moved to main draw)

• # 🇫🇷 Alizé Cornet (moved to main draw)

• # 🇦🇺 Arina Rodionova (moved to main draw)

Qualifiers
• # 🇦🇺 Olivia Gadecki

• # 🇺🇸 McCartney Kessler

• # 🇪🇸 Marina Bassols Ribera

• # Erika Andreeva

Lucky loser
• # 🇯🇵 Mai Hontama