2024 Prague Open – Singles

Nao Hibino was the reigning champion, but did not participate this year.

Seeds
• #🇨🇿 Linda Nosková

• #🇨🇿 Kateřina Siniaková

• #🇺🇦 Anhelina Kalinina

• #🇵🇱 Magda Linette

• #🇧🇬 Viktoriya Tomova

• #🇵🇱 Magdalena Fręch

• #🇦🇷 Nadia Podoroska (first round)

• #🇸🇰 Anna Karolína Schmiedlová

Seeds
• # 🇫🇷 Elsa Jacquemot (moved to main draw)

• # 🇩🇪 Ella Seidel (moved to main draw)

• # 🇺🇦 Katarina Zavatska (qualified)

• # 🇩🇪 Mona Barthel (qualified)

• # 🇦🇺 Priscilla Hon (first round)

• # 🇺🇸 Louisa Chirico (qualified)

• # 🇺🇦 Anastasiia Sobolieva (qualified)

• # 🇨🇿 Tereza Martincová (first round)

• # 🇱🇮 Kathinka von Deichmann (qualifying competition, lucky loser)

• # 🇧🇪 Alison Van Uytvanck (qualifying competition, lucky loser)

• # 🇷🇸 Dejana Radanović (first round)

• # Oksana Selekhmeteva (qualified)

Qualifiers
• # Oksana Selekhmeteva

• # 🇺🇦 Anastasiia Sobolieva

• # 🇺🇦 Katarina Zavatska

• # 🇩🇪 Mona Barthel

• # Tara Würth

• # 🇺🇸 Louisa Chirico

Lucky losers
• # 🇱🇮 Kathinka von Deichmann

• # 🇧🇪 Alison Van Uytvanck

• # 🇯🇵 Ena Shibahara