Athletics at the 1989 Summer Universiade – Women's 400 metres

The women's 400 metres event at the 1989 Summer Universiade was held at the Wedaustadion in Duisburg with the final on 25, 26, and 27 August 1989.

The winning margin was 1.68 seconds which as of 2024 remains the only time the women's 400 metres was won by more than 1.5 seconds at these games.