Ayliffe

Ayliffe is an English surname. Notable people with this surname include:


 * Graham Ayliffe (1926–2017), British microbiologist
 * John Ayliffe (1676–1732), English jurist expelled from Oxford University
 * Royce Ayliffe (born 1956), Australian professional rugby league footballer
 * Thomas Hamilton Ayliffe (1774–1852) and family, early settlers of South Australia