Bismuth sulfite agar



Bismuth sulfite agar is a type of agar media used to isolate Salmonella species. It uses glucose as a primary source of carbon. Bismuth and brilliant green (dye) both inhibit gram-positive growth. Bismuth sulfite agar tests the ability to use ferrous sulfate and convert it to hydrogen sulfide.

Bismuth sulfite agar typically contains (w/v):


 * 1.6% bismuth sulfite Bi2(SO3)3
 * 1.0% pancreatic digest of casein
 * 1.0% pancreatic digest of animal tissue
 * 1.0% beef extract
 * 1.0% glucose
 * 0.8% dibasic sodium phosphate
 * 0.06% ferrous sulfate • 7 water
 * pH adjusted to 7.7 at 25 °C

This medium is boiled for sterility, not autoclaved.