Chalgrove Brook

Chalgrove Brook is a chalk stream located in South Oxfordshire, England.

It is a left tributary of the River Thame. It rises in a number of natural springs at the foot of the Chiltern Hills near Watlington, and flows in a northwesterly direction for approximately 7 mi through Cuxham, Chalgrove, and Stadhampton, before emptying into the Thame near Chiselhampton.

The brook floods occasionally, especially in and downstream of Chalgrove. To relieve this, a man-made conduit was built in Chalgrove, splitting the stream in two.

The stream is home to brown trout, mink, otter and water vole. As part of the brook's conservation efforts, in 2023 a fish pass was created to circumvent Stadhampton Mill, making available 2 mi of the brook that was previously impassable to fish.