Coenzyme F420-1:gamma-L-glutamate ligase

Coenzyme F420-1:γ- L -glutamate ligase (, F420:gamma-glutamyl ligase, CofE-AF, MJ0768, CofE) is an enzyme with systematic name L-glutamate:coenzyme F420-1 ligase (GDP-forming). This enzyme catalyses the following chemical reaction


 * GTP + coenzyme F420-1 + L -glutamate $$\rightleftharpoons$$ GDP + phosphate + coenzyme γ-F420-2

In some instances, this enzyme is fused with a coenzyme F420-0:L-glutamate ligase domain, so that the coenzyme F420-1 produced by the latter is quickly ligated to coenzyme F420-2. This enzyme is also able to serially add additional γ-glutamyl groups to F420-2, producing F420-3 and so on.