Flossie

Flossie may refer to:
 * Flossie, a Venus of Fifteen, an 1897 English erotic novel
 * Tropical Storm Flossie (disambiguation)

People with the given name Flossie

 * Flossie Page (1893–2006), American supercentenarian
 * Flossie Wong-Staal (1947–2020), Chinese-American virologist and molecular biologist

Animals

 * Flossie (cat) (1995–), British domestic cat which is currently the oldest verified cat alive