Gladys

Gladys may refer to:


 * Gladys (given name), people with the given name Gladys
 * Gladys (album), a 2013 album by Leslie Clio
 * Gladys (film), 1999 film written and directed by Vojtěch Jasný
 * Gladys, Virginia, United States
 * Gladys the Swiss Dairy Cow, a 2002 sculpture of a cow
 * Hurricane Gladys (1968)
 * Talia Gladys, a character in the anime series Gundam Seed Destiny
 * the launch name used for USA-215, an American reconnaissance satellite
 * a character from the novel The Lost World
 * a character in the cartoon The Grim Adventures of Billy & Mandy