Glucosaminate ammonia-lyase

The enzyme Glucosaminate ammonia-lyase (EC 4.3.1.9) catalyzes the chemical reaction


 * 2-amino-2-deoxy- D -gluconate = 2-dehydro-3-deoxy- D -gluconate + NH3 (overall reaction)
 * (1a) 2-amino-2-deoxy- D gluconate = (2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate + H2O
 * (1b) (2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate = (4S,5R)-4,5,6-trihydroxy-2-iminohexanoate (spontaneous)
 * (1c) (4S,5R)-4,5,6-trihydroxy-2-iminohexanoate + H2O = 2-dehydro-3-deoxy D -gluconate + NH3 (spontaneous)

This enzyme belongs to the family of lyases, specifically ammonia lyases, which cleave carbon-nitrogen bonds. The systematic name of this enzyme class is 2-amino-2-deoxy- D -gluconate ammonia-lyase (isomerizing; 2-dehydro-3-deoxy- D -gluconate-forming). Other names in common use include glucosaminic dehydrase,  D -glucosaminate dehydratase,  D -glucosaminic acid dehydrase, aminodeoxygluconate dehydratase, 2-amino-2-deoxy- D -gluconate hydro-lyase (deaminating), aminodeoxygluconate ammonia-lyase, 2-amino-2-deoxy- D -gluconate ammonia-lyase, and  D -glucosaminate ammonia-lyase. This enzyme participates in the pentose phosphate pathway. It employs one cofactor, pyridoxal phosphate.