Guinea-Bissau at the 2020 Summer Olympics

Guinea-Bissau competed at the 2020 Summer Olympics in Tokyo. Originally scheduled to take place from 24 July to 9 August 2020, the Games were postponed to 23 July to 8 August 2021, because of the COVID-19 pandemic. It was the nation's seventh consecutive appearance at the Summer Olympics.

Competitors
The following is the list of number of competitors in the Games.

Athletics
Guinea-Bissau received universality slots from World Athletics to send two athletes (one male and one female).


 * Key
 * Note–Ranks given for track events are within the athlete's heat only
 * Q = Qualified for the next round
 * q = Qualified for the next round as a fastest loser or, in field events, by position without achieving the qualifying target
 * NR = National record
 * N/A = Round not applicable for the event
 * Bye = Athlete not required to compete in round


 * Track & road events

Judo
Guinea-Bissau qualified one judoka for the women's half-lightweight category (52 kg). Rio 2016 Olympian Taciana Cesar received an African continental quota as the nation's top-ranked judoka outside of direct qualifying position on the IJF World Ranking List of June 28, 2021.

Wrestling
Guinea-Bissau qualified two male freestyle wrestlers, both of whom progressed to the top two of their respective events at the 2021 African & Oceania Qualification Tournament in Hammamet, Tunisia.

Key:
 * VT (ranking points: 5–0 or 0–5) – Victory by fall.
 * VB (ranking points: 5–0 or 0–5) – Victory by injury (VF for forfeit, VA for withdrawal or disqualification)
 * PP (ranking points: 3–1 or 1–3) – Decision by points – the loser with technical points.
 * PO (ranking points: 3–0 or 0–3) – Decision by points – the loser without technical points.
 * ST (ranking points: 4–0 or 0–4) – Great superiority – the loser without technical points and a margin of victory of at least 8 (Greco-Roman) or 10 (freestyle) points.
 * SP (ranking points: 4–1 or 1–4) – Technical superiority – the loser with technical points and a margin of victory of at least 8 (Greco-Roman) or 10 (freestyle) points.


 * Freestyle
 * Men