Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)

In enzymology, a hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) is an enzyme that catalyzes the chemical reaction


 * 2 ATP + hydrogenobyrinic acid + 2 L -glutamine + 2 H2O $$\rightleftharpoons$$ 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L -glutamate

The four substrates of this enzyme are ATP, hydrogenobyrinic acid, L-glutamine, and H2O; its four products are ADP, phosphate, hydrogenobyrinic acid a,c-diamide, and L-glutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is hydrogenobyrinic-acid:L-glutamine amido-ligase (AMP-forming). This enzyme is also called CobB and is part of the biosynthetic pathway to cobalamin (vitamin B12) in aerobic bacteria.