Inline speed skating at the 2009 World Games – Women's 300 m time trial

The women's 300 m time trial competition in inline speed skating at the 2009 World Games took place on 17 July 2009 at the Yangming Skating Rink in Kaohsiung, Taiwan.

Competition format
A total of 23 athletes entered the competition. Best twelve athletes from preliminary round advances to the final.