James Bethune (disambiguation)

James Bethune may refer to:
 * James Beaton (or Bethune) (1473–1539), Scottish Roman Catholic bishop and Keeper of the Great Seal of Scotland
 * James Bethune (1840–1884), Canadian politician
 * James Gray Bethune (1793–1841), Canadian businessman and judge
 * James Lindesay-Bethune, 16th Earl of Lindsay (born 1955), Scottish businessman and politician