James Gillespie

James Gillespie may refer to:


 * James Gillespie (U.S. politician) (c. 1747–1805), U.S. Congressman from North Carolina
 * James Gillespie (minister) (1720–1791), Scottish minister and theologian
 * James Gillespie (footballer) (1868–1932), Scottish footballer
 * James Gillespie (philanthropist) (1726–1797), Scottish snuff and tobacco merchant
 * James Gillespie (mariner), HM Excise officer and commander of the cutter Henry Dundas
 * James J. Gillespie (1892–1959), member of the Iowa Senate
 * James U. Gillespie (died 1981), member of the Florida House of Representatives