Ketosteroid monooxygenase

Ketosteroid monooxygenase (, steroid-ketone monooxygenase, progesterone, NADPH2:oxygen oxidoreductase (20-hydroxylating, ester-producing), 17alpha-hydroxyprogesterone, NADPH2:oxygen oxidoreductase (20-hydroxylating, side-chain cleaving), androstenedione, NADPH2:oxygen oxidoreductase (17-hydroxylating, lactonizing)) is an enzyme with systematic name ketosteroid,NADPH:oxygen oxidoreductase (20-hydroxylating, ester-producing/20-hydroxylating, side-chain cleaving/17-hydroxylating, lactonizing). This enzyme catalyses the following chemical reaction


 * ketosteroid + NADPH + H+ + O2 $$\rightleftharpoons$$ steroid ester/lactone + NADP+ + H2O (general reaction)
 * (1) progesterone + NADPH + H+ + O2 $$\rightleftharpoons$$ testosterone acetate + NADP+ + H2O
 * (2) androstenedione + NADPH + H+ + O2 $$\rightleftharpoons$$ testololactone + NADP+ + H2O
 * (3) 17alpha-hydroxyprogesterone + NADPH + H+ + O2 $$\rightleftharpoons$$ androstenedione + acetate + NADP+ + H2O

Ketosteroid monooxygenase is a single FAD-containing enzyme that catalyses three types of monooxygenase reaction.